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cetp signal cetp is a member of a class of drugs search main menu skip to primary content skip to secondary content homesample page post navigation ← older posts clinical studies suggest that nsaids, particularly the highly sel posted on february 27, 2017 by admin reply clinical studies suggest that nsaids, particularly the highly selective cyclooxygenase (cox)-2 inhibitors, are promising anticancer agents. pyrimidinyl-piperazine fused with heterocyclic benzothiazole derivatives have shown an array of biological activities viz. antimicrobial anticancer and anti-inflammatory. 8 piperazines attached to benzimidazole and indole were found to have potent anti-inflammatory activity. 9 with this concept of acetamide bridge, n. m. raghavendra et al, reported the pharmacological activity of n-(benzo[d]thiazol-2-yl)-2-(piperazin-1-yl) acetamide analogs for their anti-inflammatory activity. 10 and 11 pyrimidine and fused benzothiazole heterocycles are reported to be effective pharmacophores, ahmed kamal et al synthesized pyrazolo[1,5a] pyrimidine linked 2-aminobenzothizole selleck chemicals conjugate which were evaluated for their anticancer activity against five human cancer cell lines.12 according to quantitative structure–activity relationship approach papadopoulou c et al, reported that derivatives of 4-phenyl-piperazine were found to be potent anti-inflammatory agents.13 literature review showed that benzothiazole substituted at 4 or 5 positions with electron withdrawing groups have significant anti-inflammatory activity.14 in the light of these overall observations, prompted us to synthesize a novel derivatives gsk1349572 in vitro of substituted n-(1,3-benzothiazol-2-yl)-2-[4-(5-cyano-6-imino-2-oxo-1,2,3,6-tetrahydropyrimidin-4-yl) piperazin-1-yl]acetamide, and to screen for in-vitro anti-inflammatory activity by inhibition of albumin denaturation technique and for anticancer activity at nci. in present work target compounds were obtained by reaction of starting material of bis (methylthio) methylene malononitrile with molar equivalent liothyronine sodium amount of urea in presence of toluene and triethylamine for five hrs to give compound 4-imino-6-(methylsulfanyl)-2-oxo-1,2,3,4-tetrahydropyrimidine-5-carbonitrile (1). compound (1) posses nucleophilic replaceable active methylthio group at the 6th position, which is activated by the ring 1st position nitrogen atom and the electron withdrawing cyano group at 5th position, which was substituted by piperazine ring by reacting equal molar quantities of compound (1) & piperazine to give 4-imino-2-oxo-6-(piperazin-1-yl)-1,2,3,4-tetrahydropyrimidine-5-carbonitrile (2). the formation of compound (2) was confirmed by spectral data. substituted 2-amino benzothiazoles reacted independently with chloroacetyl chloride to give substituted 2-chloroacetylamino benzothiazole (3a–3j). posted in uncategorized | leave a reply the most frequent pain outcome used was a numeric scale (n = 29) posted on february 27, 2017 by admin reply the most frequent pain outcome used was a numeric scale (n = 29). one trial reported pain outcomes using the von korff scale ( von korff et al 1990), and one trial reported the number of participants who experienced improvement in neck pain. disability outcomes were reported by 18 of the 33 eligible trials. the disability measures used included the neck disability index ( vernon and moir 1991, n = 8), northwick park neck pain questionnaire ( leak et al 1994, n = 3), million scale ( million et al 1982, n = 2), neck pain and disability index ( wheeler et al 1999, n = 2), modified whiplash disability questionnaire ( skillgate et al 2007, n = 1), and single- and multiple-item numerical scales (n = 2) ( petrie and hazleman 1986, viljanen et al 2003). selleckchem autophagy inhibitor for all interventions, pain outcomes at the conclusion of treatment are presented in figure 2 and at medium-and longterm follow-up in figure 3. for all interventions, disability outcomes at the conclusion of treatment are presented in figure 4 and at medium-and long-term follow-up in figure 5. (see also tables 3 to 6 on the eaddenda for detailed data.) medication: two trials were identified that compared the short-term analgesic effects of medications with placebo. one trial ( hoivik and moe 1983) found more effective pain relief from an 8-day course of norgesic (ie, combination orphenadrine 35mg and paracetamol 450mg) than placebo (md –17, 95% ci –32 to –2). one trial ( thomas et al 1991) found no significant difference in immediate pain relief between single doses ly294002 chemical structure of diazepam (5mg) and placebo (md –1, 95% ci –5 to 3). neither trial reported medium- or longterm outcomes. relaxation: one trial investigated relaxation ( viljanen et al 2003). this three-arm trial compared intensive relaxation training with dynamic strengthening exercise and with minimal intervention in women with chronic neck pain. there was no significant difference in pain outcomes between relaxation training and minimal intervention at the conclusion of treatment (md 2, 95% ci –4 to 8) or at medium- (md 1, 95% ci –6 to 8), parvulin or long-term (md 1, 95% ci –6 to 8) follow-up. in addition, there was no significant difference in disability outcomes between relaxation training and minimal intervention at the conclusion of treatment (md 0, 95% ci –4 to 4), medium- (md 1, 95% ci –3 to 6), or long-term (md 3, 95% ci –2 to 7) follow-up. acupuncture: five trials compared acupuncture with sham intervention. the shams used in these trials included needling procedures without skin penetration ( itoh et al 2007, nabeta and kawakita 2002) and deactivated electrotherapy devices ( petrie and hazleman 1986, vas et al 2006, white et al 2004). one trial compared acupuncture with minimal treatment ( witt et al 2006). posted in uncategorized | leave a reply in africa, data on intussusception epidemiology and clinical mana posted on february 26, 2017 by admin reply in africa, data on intussusception epidemiology and clinical management and outcome are limited, thus posing hurdles in implementing reliable postlicensure surveillance systems for monitoring safety of rotavirus vaccines. the results of this workshop of intussusception experts in africa impart several valuable lessons that advance our understanding of factors important for establishing intussusception surveillance in africa. first, the age distribution of naturally occurring intussusception cases in africa is similar to that described in other regions of the world, peaking between 3 and 9 months of age [3] and [15]. this important finding is particularly relevant for rotavirus vaccines which are administered orally at this website ages during infancy when the intussusception rates are drastically changing. the learn more background rates of intussusception are lowest during the first 3 months of life and then increase 8–10 fold between 4 and 6 months of age. this period of infant life also coincides with the time when routine vaccines are administered in africa. because rotavirus vaccines are orally administered, cases of intussusception that bear a temporal relationship with vaccination (e.g., within 1–2 weeks of vaccine receipt) might be falsely attributed as associated with the vaccine. thus, to minimize the number of intussusception cases that are temporally associated with the first dose of vaccine, when risk of intussusception is theoretically greatest (based on the rotashield® experience) and peak timing of vaccine virus replication in the gut, the who recommends that rotavirus vaccines be initiated by 15 weeks of age [16]. however, in africa, nearly 20–25% of the infants typically present after 15 weeks of life for their first routine epi visit [17] and [18]. thus delays in rotavirus vaccination are likely and could lead to a greater number of intussusception events that are temporally linifanib (abt-869) linked to vaccine administration whether causal or not. this highlights the need for careful monitoring of intussusception events through robust surveillance and epidemiologic studies to disentangle a causal association from spurious chance findings. the distinct age epidemiology of intussusception in africa will need to be an important consideration for analysis of data yielded through any intussusception surveillance system. secondly, the observed data from many of the countries included in this analysis, do not demonstrate a seasonal nature to the peak of intussusception cases. this is of interest because in many of these countries, robust rotavirus surveillance over a number of years has demonstrated the seasonal occurrence of rotavirus associated hospitalizations due to acute infantile gastroenteritis [19] and [20]. posted in uncategorized | leave a reply for each included patient we recorded sex, age at death, and time posted on february 26, 2017 by admin reply for each included patient we recorded sex, age at death, and time between last visit and death. for patients in the data at diagnosis group (423/592, 71.5%) we also noted type of glaucoma (poag or pexg), age at diagnosis, and years with a glaucoma diagnosis. the presence of exfoliation syndrome (pex) was recorded if noted at the time of diagnosis or up to 1 year azd6738 supplier later. in addition, all available data were reviewed to clarify if pex had been documented in eyes that had undergone cataract surgery before the glaucoma diagnosis was established. a diagnosis of glaucoma required that at least 1 eye: (1) showed a repeatable visual field defect (vfd) consistent with glaucoma and not explained by other causes; or (2) had only 1 visual field test but with a vfd consistent with glaucoma and a corresponding optic disc abnormality; or (3) was already blind (visual acuity abt-888 cell line had a record of a totally cupped glaucomatous optic disc. patients were excluded if other disease made it impossible to establish a glaucoma diagnosis with certainty or to determine whether the visual field showed glaucomatous field loss or not (eg, patients with optic disc drusen or endocrine ophthalmopathy). patients were routinely followed with standard automated perimetry using the humphrey perimeter (carl zeiss meditec, dublin, california, usa) 30-2 or 24-2 full-threshold or sita programs. visual field defects were defined as glaucomatous if they showed a pattern consistent with glaucoma (eg, a nasal step or a paracentral or arcuate defect). in addition, the glaucoma hemifield test (ght) had to be classified as “borderline” or “outside normal limits.” visual fields were considered reliable electron transport chain if false-positive responses were fewer than 15% and a clear blind spot could be seen in the visual field printouts (threshold value posted in uncategorized | leave a reply lymph nodes from vaccinated animals showed statistically signific posted on february 25, 2017 by admin reply lymph nodes from vaccinated animals showed statistically significantly lower bacterial counts at weeks 2 (ρ = 0.0107) and 3 (ρ = 0.0439) compared to lymph nodes from control animals after challenge. at week 2, the bacterial load in the right prescapular lymph nodes of naïve cattle ranged from 3.954 log10 cfu to 5.838 log10 cfu with a median of 5.431 log10 cfu; in the right prescapular lymph nodes from ku-57788 supplier bcg-vaccinated cattle counts ranged from 2.041 log10 cfu to 5.38 log10 cfu with a median of 4.688 log10 cfu. at three weeks, the bacterial load in the right prescapular lymph node of naïve cattle ranged from 3.587 log10 cfu to 5.068 log10 cfu with a median of 4.648 log10 cfu; in the right prescapular lymph nodes from bcg-vaccinated cattle counts ranged from 2.591 log10 cfu to 4.944 log10 ch5424802 purchase cfu with a median of 3.8 log10 cfu. the number of bcg cfu recovered from naïve animals at week 2 was higher than the cfu recovered at week 3; this difference was statistically significant (ρ = 0.0109). on the other hand, no difference was found in bcg cfu recovered at week 2 compared to week 3 in bcg vaccinated animals. it was of interest to determine the distribution of the bacteria following challenge with bcg-tokyo. to that effect, as well as evaluating bacterial counts in the right prescapular lymph nodes, counts were also evaluated in left prescapular lymph nodes and in left and right submandibular and popliteal lymph nodes. table 1 shows the proportion of animals presenting bacterial counts in the different lymph nodes according to time and treatment. the data indicate that the dissemination of bcg tokyo was greater in naïve control animals compared to animals that had been vaccinated with bcg at week 0. the differences at both 2 and 3 weeks were statistically significant (ρ = 0.0017 and ρ = 0.0005, respectively). vaccination and challenge experiments are a necessity for the development of vaccines against bovine tb. however, these experiments involve the use of large animal bsl3 facilities. whilst necessary, due to their nature, these facilities are expensive to run and limited in number and therefore represent a bottle neck for the testing of vaccine candidates. development oxalosuccinic acid of a model in the target species, cattle, for prioritizing vaccines under lower containment conditions would save money as bsl2 facilities are cheaper to run than bsl3 facilities. being an attenuated strain of m. bovis it would be expected that cattle would at some stage control bcg and therefore the bcg challenge experiments would be shorter than standard virulent m. bovis challenge experiments. further, by reducing the need for bsl3 experimentation, vaccine development programmes could be significantly accelerated. posted in uncategorized | leave a reply e state or locally hired distributors) for further distribution posted on february 25, 2017 by admin reply e. state or locally hired distributors) for further distribution to small providers, and the estimated proportion of doses bvd523 that were administered in public sites. two factors were related to existing health infrastructure: the maximum number of ship-to-sites had a positive association with coverage, and the percentage of medically underserved population a negative association. coverage was also negatively associated with population factors including the percentage of the population that will not visit a medical doctor because of cost, the number of vehicles per capita, and the percentage of population under 18 years old. for high-risk adults, two supply chain processes were positively associated with uptake: the percentage of doses shipped to “general public” locations, and the use of pharmacy and retail locations for vaccination; and one, the expansion of vaccination to the general public by december 4th, was negatively associated. coverage was positively associated with population and health related factors: percentage of women with a pap smear, past seasonal influenza rgfp966 purchase vaccination, and percentage of population that is american indian. two infrastructure factors were associated: the proportion of the population medically underserved (negatively) and the maximum number of ship-to-sites (positively). we sought to identify factors related to vaccination program decisions and processes that may have facilitated or hindered vaccine uptake for two target groups for vaccination: children and high-risk adults. several supply chain and system factors were associated with vaccination coverage of children and of high-risk adults. with the exception of the maximum number of ship-to sites, a factor that was also associated with overall adult coverage [3], factors associated with coverage of children and of high-risk dipeptidyl peptidase adults did not overlap. additionally, factors not related to program decisions such as health-seeking behaviors and population characteristics were also associated with state-to-state variation, as would be expected given baseline variation in vaccination coverage for recommended vaccines [4] and the variety of factors associated with vaccinations, both for high-risk individuals [15], [17], [18] and [33] and children [13] and [14]. several findings were related to the type of providers or locations to which vaccine was directed. for children, having a focus on school vaccination was associated with higher coverage (five of the six states that achieved the highest coverage in children implemented statewide school vaccination programs [2] and [6]), as was distribution to public sites. public sites can include schools, but also locations such as mass clinics run by health departments. for high-risk adults, more distribution to providers with a broad base of access (including pharmacies, primary care providers, county health departments, etc.) was associated with higher coverage. posted in uncategorized | leave a reply more broadly, it may be important to intentionally address the ro posted on february 24, 2017 by admin reply more broadly, it may be important to intentionally address the role of non-occupational physical activity within groups of people with increasingly mechanized jobs. study design and population. the saskatchewan farm injury cohort study (sfic) was developed to understand more about the health of farm populations (pickett et al., 2008). it involved development of a diverse sample of farms in order to study relationships between individual and contextual factors and health outcomes. the present study was based on baseline data from phase 2 of the sfic, which was initiated in january 2013. the sample consisted of 2,849 individuals (2,619 adults) residing and/or working on 1,216 farms from hif inhibitor 74 different rural municipalities. participation rates were 93% at the municipality level and 48% at the farm level. a health and operational survey was sent by mail and completed by a single informant on each farm. information was collected about each farm resident and farm operation. the dillman total design method for self-administered questionnaires was utilized (dillman, 2000). survey procedures were tested via a pilot trial (day et al., 2008) as described elsewhere (pickett et al., 2008). informed consent was indicated by completion and return of the questionnaire. the study was approved by the behavioural research ethics board of the university of saskatchewan. study variables overweight and obesity. respondents reported each participant’s weight (in pounds or kilograms) and height (in feet and inches, or cm) which were used to calculate the body mass index (bmi, kg/m2). bmis were separated anti-diabetic compound library datasheet into non-overweight, overweight, and obese categories using standardized thresholds for adults ( “parent”, “child”, adenylyl cyclase “other relative”); level of formal education completed (“less than high school”, “completed high school”, “completed university”, “technical/community college”); reports of an off-farm occupation (“none”, “part-time”, “full-time”) (statistics canada, 2014); and number of reported comorbidities (0, 1, ≥ 2). we also asked about health behaviors: alcohol consumption in the previous year (4 categories: “never” through “more than once a week”) (statistics canada, 2013); excessive daytime sleepiness (> 10 on the epworth sleepness scale) (johns and hocking, 1997); and current smoking status (“yes” or “no”) (statistics canada, 2013). farm-level covariates. farm factors considered were estimated total farm acreage (“≤500”, “501-1500”, “1501-2500”, “>2500”); commodities produced (e. posted in uncategorized | leave a reply heart rate was significantly lower in the triple noss null than i posted on february 24, 2017 by admin reply heart rate was significantly lower in the triple noss null than in the wild-type mice, and the degree of bradycardia in the triple noss null mice was also equivalent to that in the enos gene-disrupted single and double noss null mice (fig. 1b), indicating that bradycardia is also a common phenotype of the enos gene deletion. although there is no conclusive explanation for the decreased heart rate in association with the enos gene deletion, previous studies revealed that enos-derived no could affect baroreflex resetting or could be involved in establishing the parp phosphorylation baroreceptor setpoint (31). we previously revealed that not only enos and inos but also nnos is expressed in vascular lesions in a mouse carotid artery ligation model and a rat balloon injury model, and that all three noss play a role in the regulation of vascular lesion formation (7), (8), (9) and (32). spontaneous development doxorubicin ic50 of vascular lesion formation (neointimal formation, medial thickening, and perivascular fibrosis) was noted in the large epicardial coronary arteries, coronary microvessels, and renal arteries in the triple noss null mice, but not in the enos null mice (2) and (33). spontaneous lipid accumulation was also observed in the aorta of the triple noss null mice (2) and (33). these results suggest the crucial role of noss in inhibiting vascular lesion formation. the extent of hypertension was comparable in the triple noss null and enos null mice, whereas spontaneous vascular lesion formation was observed only in the triple noss null mice, suggesting a minor role of hypertension in vascular lesion formation in the triple noss null mice (2) and (33). mycoclean mycoplasma removal kit bone marrow-derived vascular progenitor cells in the blood accumulate in injured arteries, differentiate into vascular wall cells, and contribute to arteriosclerotic vascular lesion formation. all noss have been reported to be expressed in bone marrow cells. however, whether noss in bone marrow cells play a role in vascular lesion formation remained to be clarified. we previously reported that, in wild-type mice that underwent bone marrow transplantation from green fluorescent protein-transgenic mice, green fluorescent protein-positive fluorescence was detected in the ligated carotid arteries, confirming the involvement of bone marrow-derived vascular progenitor cells in vascular lesion formation after carotid artery ligation (34). in a comparison between the triple noss null genotype that received the triple nos null bone marrow transplantation and the triple noss null genotype that received the wild-type bone marrow transplantation, the extent of neointimal formation and the extent of constrictive remodeling were both significantly less in those that received the wild-type bone marrow transplantation, along with significantly higher nos activities in the ligated carotid arteries (fig. 2) (35). posted in uncategorized | leave a reply amount of keto, mp and pp in sample was calculated by comparing t posted on february 23, 2017 by admin reply amount of keto, mp and pp in sample was calculated by comparing the mean rf for standard and sample solution by formula no. 2. amount of keto, mp and pp in sample (mg) was calculated by following formula: equation(2) amountofdrugketo,mpandpp(ml)estimated(mg)=meanamountestimated(μg)inappliedvolumevolumeofsamplesolutionapplied(μl)×volumeofstocksolution amount of the drug recovered (mg) and % recovery was sb203580 solubility dmso calculated and results of recovery studies and statistically are shown in table 4 and table 5. intra-day precision was determined by analyzing gel sample solutions at different time intervals on the same day. gel sample solution was prepared and analyzed in the similar manner as described under analysis of the gel formulation. inter-day precision was determined by analyzing gel sample solutions on three different days. gel sample solution was prepared and analyzed in the similar manner as described in analysis of the gel formulation. results of intra-day precision and inter-day precision are shown in table 6 and table 7, respectively. the lod and loq were separately determined which is based on the standard deviation of response of the calibration curve. the standard deviation of y-intercept and slope of the calibration curves were used to calculate the lod and loq. results are shown in table 8. to evaluate the robustness of the proposed method, small but deliberate variations in the optimized method parameters were done. the effect of change in flow rate and mobile phase ratio on retention time and tailing factor were studied. the solution containing 25 μg/ml of keto, 12.5 μg/ml of mp and 0.5 μg/ml of pp was injected (in triplicate) ps 341 into sample injector of hplc three times under the varied conditions. robustness data is given in table 9. amount of gel equivalent to about 25 mg keto was separately transferred to five different 25.0 ml volumetric flasks (flask no. 1, 2, 3, 4 and 5), added 5.0 ml of 0.1 m hcl, 0.1 m naoh and 3% h2o2 to flask no. 1, 2 and 3, respectively. solution in flask no. 1, 2, and 3 were heated in water bath for 3 h at 80 °c. flask no. 4 containing gel was kept at 60 °c for 24 h to study the effect of heat on gel sample (heat degradation). the forced degradation was performed in the dark to exclude the possible degradative effect of light. flask no. 5 was exposed to ultraviolet radiations isothipendyl at 254 nm for 24 h in a uv-chamber. all the flasks were removed gel samples were treated and analyzed in similar manner as described under analysis of gel formulation. the typical densitogram is shown in fig. 9, fig. 10, fig. 11, fig. 12 and fig. 13for acidic, alkaline, oxide, heat and uv exposure, respectively. results of forced (stress) degradation studies are shown in table 10. in the present work, new method namely, simultaneous equation method and quick high-performance liquid chromatography (hplc) method were developed and validated for the simultaneous determination of three compounds in a formulated gel. posted in uncategorized | leave a reply the collected samples were stored at 4 °c starch degrading micro posted on february 23, 2017 by admin reply the collected samples were stored at 4 °c. starch degrading microbes were isolated using strach agar medium (sam). the isolates showing maximum clear halo zone were sub-cultured.7 selective isolates with maximum starch degrading activities were identified up to species level.8 and 9 the most potent isolates were finally chosen for further studies. the inoculum for further enzyme modulation and other studies was prepared using luria broth (lb) medium. the fresh overnight culture was used as an inoculum for the production of amylase.10 the inoculated medium was incubated at 37 °c for 48 h by shake flask fermentation method at 200 rpm. the culture broth was then centrifuge at 8000 × g 10 min at 4 °c. the free cell supernatant bibw2992 was used as an extracellular crude enzyme. 11 total protein concentrations were determined by bradford’s method using bovine serum albumin (bsa) as the protein standard.12 α-amylase activity was determined by measuring the formation of reducing sugars released during starch hydrolysis. the amount of liberated reducing sugar was determined by dinitrosalicylic acid (dns) method. glucose was used to construct this website the standard curve.4 five percent bacterial inoculum was added aseptically to 500 ml of sterile growth medium and incubated at 37 °c at 150 rpm. twenty ml of culture was taken periodically for 48 h at every 6 h intervals. the amylase activity was determined in the culture filtrate. the effect of ph on amylase activity was determined at different ph (6.5, 7, 7.5, 8, 8.5 and 9) and the effect of temperature on enzyme activity was determined using different temperature (26 °c, 29 °c, 32 °c, 35 °c, 38 °c and 41 °c).11 different carbon and nitrogen sources (both at concentration of 10 g/l) were used in minimal medium, ph 7 and incubated at 32 °c for 24 h. similarly different amino acids like glycine, alanine, aspartic acid and cysteine were used in the medium for optimization.13 the culture filtrates were assayed for total protein content else and amylase activity. the culture filtrate was precipitated using 80% w/v ammonium sulfate precipitation method.14 then the precipitate was separated by centrifugation at around 6700 × g for 10 min. the pretreatment of the dialysis membrane was done ashwini et al, 2011. genomic dna was extracted using phenol–chloroform extraction method. the pcr parameters for the amplification of 16s ribosomal dna were optimized. 50 μl of pcr master mix contained universal primer set 27 f- (5′-ag agt ttg atc mtg gct cag-3′)/1492 r- (5′-g gyt acc ttg tta cga ctt-3′), 10 mm dntps, 10× pcr buffer, 1 u taq dna polymerase, 2 mm mg+ and (100–200 ng) template dna. pcr steps included initial denaturation at 95 °c for 5 min, 35 cycles of denaturation at 95 °c for 1 min, annealing at 56 °c for 2 min, elongation at 72 °c for 1 min and final extension at 72 °c for 10 min. approximately 1.5 kb amplicons were generated. posted in uncategorized | leave a reply post navigation ← older posts search recent posts clinical studies suggest that nsaids, particularly the highly sel the most frequent pain outcome used was a numeric scale (n = 29) in africa, data on intussusception epidemiology and clinical mana for each included patient we recorded sex, age at death, and time lymph nodes from vaccinated animals showed statistically signific recent commentsmr wordpress on hello world!archives february 2017 january 2017 december 2016 november 2016 october 2016 september 2016 august 2016 july 2016 june 2016 may 2016 april 2016 march 2016 february 2016 january 2016 december 2015 november 2015 october 2015 september 2015 august 2015 june 2015 may 2015 april 2015 march 2015 february 2015 january 2015 december 2014 november 2014 october 2014 september 2014 august 2014 july 2014 june 2014 may 2014 april 2014 march 2014 february 2014 january 2014 december 2013 november 2013 october 2013 september 2013 august 2013 july 2013 june 2013 may 2013 april 2013 march 2013 february 2013 january 2013 categories uncategorized meta log in entries rss 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